Secondary ossification center induces and protects growth plate structure

Author(s)
Meng Xie, Pavel Gol'din, Anna Nele Herdina, Jordi Estefa, Ekaterina V Medvedeva, Lei Li, Phillip T Newton, Svetlana Kotova, Boris Shavkuta, Aditya Saxena, Lauren T Shumate, Brian D Metscher, Karl Großschmidt, Shigeki Nishimori, Anastasia Akovantseva, Anna P Usanova, Anastasiia D Kurenkova, Anoop Kumar, Irene Linares Arregui, Paul Tafforeau, Kaj Fried, Mattias Carlström, András Simon, Christian Gasser, Henry M Kronenberg, Murat Bastepe, Kimberly L Cooper, Peter Timashev, Sophie Sanchez, Igor Adameyko, Anders Eriksson, Andrei S Chagin
Abstract

Growth plate and articular cartilage constitute a single anatomical entity early in development but later separate into two distinct structures by the secondary ossification center (SOC). The reason for such separation remains unknown. We found that evolutionarily SOC appears in animals conquering the land - amniotes. Analysis of the ossification pattern in mammals with specialized extremities (whales, bats, jerboa) revealed that SOC development correlates with the extent of mechanical loads. Mathematical modeling revealed that SOC reduces mechanical stress within the growth plate. Functional experiments revealed the high vulnerability of hypertrophic chondrocytes to mechanical stress and showed that SOC protects these cells from apoptosis caused by extensive loading. Atomic force microscopy showed that hypertrophic chondrocytes are the least mechanically stiff cells within the growth plate. Altogether, these findings suggest that SOC has evolved to protect the hypertrophic chondrocytes from the high mechanical stress encountered in the terrestrial environment.

Organisation(s)
Department of Evolutionary Biology
External organisation(s)
Karolinska Institute, National Academy of Sciences of Ukraine (NASU), Medizinische Universität Wien, Uppsala University, I.M. Sechenov First Moscow State Medical University, Karolinska University Hospital, Semenov Institute of Chemical Physics (RA), Univ Calif San Diego, University of California System, University of California San Diego, Ctr Magnet Res, Harvard University, KTH - Royal Institute of Technology, European Synchrotron Radiation Facility ESRF, Lomonosov Moscow State University (MSU), UPMC Univ Paris 06, Centre National de la Recherche Scientifique (CNRS), Sorbonne Universite, Sorbonne Univ, CNRS, Lab Oceanog Villefranche LOV, Russian Academy of Sciences
Journal
eLife
Volume
9
ISSN
2050-084X
DOI
https://doi.org/10.7554/eLife.55212
Publication date
10-2020
Peer reviewed
Yes
Austrian Fields of Science 2012
106054 Zoology
Portal url
https://ucris.univie.ac.at/portal/en/publications/secondary-ossification-center-induces-and-protects-growth-plate-structure(a95a7035-ffdc-40e5-8b36-4cb0666496e4).html