A fiber-deprived diet disturbs the fine-scale spatial architecture of the murine colon microbiome

Author(s)
Alessandra Riva, Orest Kuzyk, Erica Forsberg, Gary Siuzdak, Carina Pfann, Craig Herbold, Holger Daims, Alexander Loy, Benedikt Warth, David Berry
Abstract

Compartmentalization of the gut microbiota is thought to be important to system function, but the extent of spatial organization in the gut ecosystem remains poorly understood. Here, we profile the murine colonic microbiota along longitudinal and lateral axes using laser capture microdissection. We found fine-scale spatial structuring of the microbiota marked by gradients in composition and diversity along the length of the colon. Privation of fiber reduces the diversity of the microbiota and disrupts longitudinal and lateral gradients in microbiota composition. Both mucus-adjacent and luminal communities are influenced by the absence of dietary fiber, with the loss of a characteristic distal colon microbiota and a reduction in the mucosa-adjacent community, concomitant with depletion of the mucus layer. These results indicate that diet has not only global but also local effects on the composition of the gut microbiota, which may affect function and resilience differently depending on location.

Organisation(s)
Department of Microbiology and Ecosystem Science, Department of Physiological Chemistry, Department of Food Chemistry and Toxicology
External organisation(s)
Scripps Research Institute, San Diego State University, Universität Wien
Journal
Nature Communications
Volume
10
No. of pages
11
ISSN
2041-1723
DOI
https://doi.org/10.1038/s41467-019-12413-0
Publication date
09-2019
Peer reviewed
Yes
Austrian Fields of Science 2012
Molecular biology, Microbiology, Medical biochemistry
Keywords
Portal url
https://ucris.univie.ac.at/portal/en/publications/a-fiberdeprived-diet-disturbs-the-finescale-spatial-architecture-of-the-murine-colon-microbiome(7ca8977a-a0ee-4e0e-8e1a-0e0407762d3c).html