Dental cell type atlas reveals stem and differentiated cell types in mouse and human teeth

Author(s)
Jan Krivanek, Ruslan A Soldatov, Maria Eleni Kastriti, Tatiana Chontorotzea, Anna Nele Herdina, Julian Petersen, Bara Szarowska, Marie Landova, Veronika Kovar Matejova, Lydie Izakovicova Holla, Ulrike Kuchler, Ivana Vidovic Zdrilic, Anushree Vijaykumar, Anamaria Balic, Pauline Marangoni, Ophir D Klein, Vitor C M Neves, Val Yianni, Paul T Sharpe, Tibor Harkany, Brian D Metscher, Marc Bajénoff, Mina Mina, Kaj Fried, Peter V Kharchenko, Igor Adameyko
Abstract

Understanding cell types and mechanisms of dental growth is essential for reconstruction and engineering of teeth. Therefore, we investigated cellular composition of growing and non-growing mouse and human teeth. As a result, we report an unappreciated cellular complexity of the continuously-growing mouse incisor, which suggests a coherent model of cell dynamics enabling unarrested growth. This model relies on spatially-restricted stem, progenitor and differentiated populations in the epithelial and mesenchymal compartments underlying the coordinated expansion of two major branches of pulpal cells and diverse epithelial subtypes. Further comparisons of human and mouse teeth yield both parallelisms and differences in tissue heterogeneity and highlight the specifics behind growing and non-growing modes. Despite being similar at a coarse level, mouse and human teeth reveal molecular differences and species-specific cell subtypes suggesting possible evolutionary divergence. Overall, here we provide an atlas of human and mouse teeth with a focus on growth and differentiation.

Organisation(s)
Department of Evolutionary Biology
External organisation(s)
Medizinische Universität Wien, Masaryk University, Harvard University, Karolinska Institute, University of Connecticut, King's College London, Aix-Marseille Université, Czech Academy of Sciences, University of Helsinki, University of California, San Francisco
Journal
Nature Communications
Volume
11
ISSN
2041-1723
DOI
https://doi.org/10.1038/s41467-020-18512-7
Publication date
09-2020
Peer reviewed
Yes
Austrian Fields of Science 2012
106010 Developmental biology
Keywords
Portal url
https://ucris.univie.ac.at/portal/en/publications/dental-cell-type-atlas-reveals-stem-and-differentiated-cell-types-in-mouse-and-human-teeth(5c440d8e-3f4a-4568-b3d1-7e0c900b9764).html